Journal: CNS Neuroscience & Therapeutics

Article Title: Depletion of HSP60 in Microglia Leads to Synaptic Dysfunction and Depression‐Like Behaviors Through Enhanced Synaptic Pruning in Male Mice

doi: 10.1111/cns.70394

Figure Lengend Snippet: Detailed antibody information.

Article Snippet: DrebrinA , Cell signaling technology , 12,243 , 1/1000.

Techniques:

Journal: CNS Neuroscience & Therapeutics

Article Title: Depletion of HSP60 in Microglia Leads to Synaptic Dysfunction and Depression‐Like Behaviors Through Enhanced Synaptic Pruning in Male Mice

doi: 10.1111/cns.70394

Figure Lengend Snippet: The deletion of HSP60 caused synaptic deficits and modified relative signaling pathways in the mouse cortex. (A) Spine density and column graph of Golgi staining in the mouse cortex. n = 5. (B, C) The Glu (B), GABA (C) level in the cortex of control and HSP60 cKO mice. (D–F) Representative western blotting images of NR2A, NR2B, NR2C, NR2D, AMPAR1, AMPAR2, DrebrinA, Synaptophysin, SNAP25, Synapsin‐1, PSD95 and their bar graph in the mouse cortex. (G) The Volcano plots of significantly different proteins in the cortex of control mice and HSP60 cKO mice. (H) GO enrichment analysis of significantly different proteins in the cortex of control and HSP60 cKO mice. n = 6. Mean ± SEM, Unpaired t test. ns, no significance; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: DrebrinA , Cell signaling technology , 12,243 , 1/1000.

Techniques: Modification, Protein-Protein interactions, Staining, Control, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor

doi: 10.3389/fphar.2024.1422693

Figure Lengend Snippet: Detailed antibody information.

Article Snippet: p-IP3 , Cell Signaling Technology , #12243 , 1:1000.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor

doi: 10.3389/fphar.2024.1422693

Figure Lengend Snippet: Bmal1 +/− mice show abnormal neurotransmitter levels and molecular signals. (A–F) Levels of neurotransmitters in the PFC of Bmal1 +/+ and Bmal1 +/− mice after cued fear conditioning paradigm. 5-HT (A) , dopamine (B) , GABA (C) , glutamate (D) , norepinephrine (E) , and acetylcholine (F) . n = 7 mice. Data are reported as mean ± SEM. Unpaired t-test. ns, no significance; *p < 0.05; **p < 0.01. (G, H) Representative Western blot images (G) and protein levels of IDO, 5-HT2CR, SIRT1, TPH2, TH, DAT, p-IP3, p-PKC, and p-CREB in the PFC of Bmal1 +/+ and Bmal1 +/− mice after the cued fear conditioning paradigm (H) . n = 4–6 mice. Data are reported as mean ± SEM. Unpaired t-test. ns, no significance; *p < 0.05; **p < 0.01.

Article Snippet: p-IP3 , Cell Signaling Technology , #12243 , 1:1000.

Techniques: Western Blot